There are thousands of consumables in modern laboratories, and those familiar and frequently used ones may be pipe consumables, such as centrifugal pipes. Centrifuge tubes, as the name suggests, are mainly used in conjunction with laboratory centrifuges and are inevitable consumables.
It can be divided into plastic centrifuge tubes, glass centrifuge tubes and stainless steel centrifuge tubes.
The centrifuge tubes commonly used in the laboratory are plastic and glass, and generally plastic ones are used frequently because glass centrifuge tubes cannot be used in high-speed or ultra-high-speed centrifuges. Plastic centrifugal pipes are also made of PP (polypropylene), PC (polycarbonate), PE (polyethylene) and other materials. The pipe performance of PP is relatively good. The plastic centrifuge tube is transparent or translucent, which can visually see the centrifugation of the sample, but it is relatively easy to deform and has poor corrosion resistance to organic solvents, so its service life is short. Therefore, laboratories often purchase centrifuge tubes.
PP (polypropylene): translucent has good chemical and temperature stability, but it will become brittle at low temperature, so it should not be below 4 ℃ when centrifuging.
1. PC (polycarbonate): it has good transparency and high hardness. It can be disinfected at high temperatures, but it is not resistant to strong acids, strong alkalis and some organic solvents such as alcohol. It is mainly used for ultra-high speed centrifugation with more than 50000 revolutions.
2. PE (polyethylene): opaque. It does not react with acetone, acetic acid, hydrochloric acid, etc and is relatively stable. It is easy to soften under high temperatures.
3. PA (polyamide): this material is a polymer made of PP and PE. It is translucent and has very stable chemical properties, but it is not resistant to high temperatures.
4. PS (polystyrene): PS is transparent, hard, and stable to most aqueous solutions, but it will be corroded by a variety of organic substances. It is mostly used for low-speed centrifugation and is generally disposable.
5. PF (polyfluoride): translucent can be used at low temperatures. If it is an experimental environment at -100 ℃ -140 ℃, you can use this kind of centrifugal tube.
6. Cab (butyl acetate cellulose): It is transparent and can be used for the gradient determination of dilute acids, bases, salts, alcohol and sucrose.
Microcentrifuge tube, small capacity centrifuge tube and large capacity centrifuge tube. Generally speaking, centrifuge tubes with a capacity greater bigger than 100ml are called centrifuge bottles. According to the capacity of centrifugal pipe, generally, the capacity of centrifugal pipe is divided into several main specifications, such as 1.5ml, 2ml, 5ml, 10ml, 15ml, 50ml, etc. The more commonly used ones are 10ml and 50ml.
1. A large number of centrifuge tubes (500ml, 250ml), also known as centrifuge bottles, are centrifuge options for large-capacity cell harvesting, granulation and protein purification;
2. Ordinary centrifuge tube (50ml, 15ml), a common centrifuge tube in the laboratory;
3. Microcentrifuge tubes (2ml, 1.5ml, 0.65ml, 0.2ml) are often used together with micro centrifuges for the separation of micro reagents and are often used in micro-operation experiments of molecular biology.
Other specifications include 30ml and 7ml, which are mainly suitable for different centrifuges.
According to the shape, the centrifugal tube can also be divided into the round bottom and sharp bottom, as well as a screw cover and plug-in cover. The centrifugal tube of the screw cover has a fine scale, and there is only an overall capacity mark for the plug.
1. Round bottom centrifugal tube: when collecting density gradient, the round bottom can be selected; The area of the round bottom is large, which can bear greater centrifugal force than the sharp bottom;
2. Tip bottom centrifuge tube: when there are few samples or collecting sediment, it is better to separate with a tip bottom centrifuge tube, and the upper liquid is easier to be absorbed with a straw.
3. Flat bottom centrifuge tube: the purpose is the same as that of the sharp bottom centrifuge tube, but the centrifuge tube can be erected, which is more convenient to put and take in the experiment. Other flat-bottom centrifuge tubes are mostly centrifuge bottles and sample tubes.
The covers of centrifugal pipes are generally connected with covers for centrifugal pipes with small capacity, while centrifugal pipes with ordinary capacity have covers, plugs and screw caps. Centrifugal pipes with a capacity of more than 50ml are basically screwed caps.
Generally speaking, the centrifugal pipe with cover has a marked scale on the pipe wall, the centrifugal pipe with screw cover has a finer scale, and the plug has only an overall capacity mark.
Preparation centrifuge tube and analysis centrifuge tube.
There is no strict classification standard, and the purpose of preparation type is to separate and purify biochemical substances, cells and subcellular particles. Analytical type requires higher requirements.
Low-speed centrifuge tube and high-speed centrifuge tube.
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