Plasma and Serum are both components of Blood. They are the components of the liquid blood portion. Therefore, if the blood's constituent cells, such as the Red and White Blood Cells, were to be eliminated, what would be left would be a liquid-like material that contained both Plasma and Serum. They are the medium in which Blood Cells float. But that is where the similarities between Plasma and Serum end because they are part of the same mixture but are significantly distinct.
What is blood?
Blood is a connective tissue made up of free-floating cells and fluids. It moves via blood vessels and does the duty of carrying nutrients, hormones, enzymes, oxygen, and carbon dioxide to specific cells in our body. By interacting with the acids and bases found within the human body, blood also contributes to the management of our body’s PH level and regulates body temperature. The waste that cells produce is also released into the blood and gets extracted by the kidneys.
What is serum?
The serum is the liquid portion of blood that is free of blood cells and clotting components. It develops during blood coagulation, during which the blood loses the coagulation protein fibrinogen and transforms it into the protein fibrin, which aids in wound healing. All proteins other than those necessary for blood clotting, antibodies, antigens, enzymes, hormones, electrolytes, and exogenous substances like drugs and microorganisms are all found in serum. Serum can be used to diagnose issues with things like cholesterol, blood pressure, blood sugar, etc.
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Golden VacTM SST serum separator tube gold-top
What is plasma?
Since plasma accounts for up to 55% of the blood volume, it is the essential component of blood. It is the medium in which all different types of blood cells are suspended and allowed them to float freely while performing their various functions, such as the transfer of oxygen by RBCs and the defense against alien bacteria by WBCs.
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How do separate serum and plasma form blood?
The serum is the liquid component of whole blood obtained after the blood has clotted. By utilizing centrifugation to separate the clot, the serum-designated supernatant is then carefully extracted with a Pasteur pipette. When entire blood is drawn into tubes and given an anticoagulant treatment, plasma is formed. Blood in the plasma tube does not clot. The centrifugation process separates the cells. Using a Pasteur pipette, the supernatant, referred to as plasma, is carefully separated from the cell pellet.
Differences Between Serum and Plasma
Water, clotting factors, blood cells
Water, all proteins of plasma except clotting factors
Blood Cells, Salts, Lipids, Proteins, and Glucose
Electrolytes, Antigens, Antibodies, Protein, and Hormones
Method of separation
It is acquired after centrifuging the blood with the anti-coagulant.
It is acquired by centrifuging the blood after it has coagulated.
It does not contain as it changed into Fibrin Protein.
55% of blood volume
Less in volume than plasma
Blood collection tubes
·Coagulation Tubes (Blue top tubes)
·Heparin Blood Collection Tubes(Green top tubes)
·Glucose Test Tubes (Grey top tubes)
·EDTA Tubes (Purple lavender top tubes with the gel)
Clot Activator Tubes
(Red top tubes)
·Serum Separation Tubes(SST Tube)
(Yellow top or Gold top tubes)
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