To say that the laboratory is an indispensable equipment, the centrifuge must be on the list. The lab centrifuge is easy to operate, and almost everyone is familiar with it. However, some bad or wrong operating habits may reduce the service life of the centrifuge, and seriously even endanger the safety of experimenters. So, what are the common precautions in the daily operation of the centrifuge?
The centrifuge tubes and centrifuge buckets must be placed symmetrically in the rotor, and the centrifuge tubes and their contents must be balanced on the balance. Asymmetrical placement will lead to uneven force on the shaft during centrifugation, resulting in wear and tear, reducing the service life of the centrifuge. Severe cases may even cause the centrifugal rotor to fly out of the centrifugal cavity. Imagine the destructive power of thousands of rotors. Therefore, in order to prolong the service life of the centrifuge and for the safety of the experimenters, it must be balanced before centrifugation.
It is also possible that due to the long-term use of the balance without calibration, the imbalance of the symmetrically placed centrifuge tubes exceeds the allowable sample weighing error of the centrifuge.
When using the swinging rotor, the serial number of the hanging basket and the main body of the swinging rotor are accidentally installed incorrectly, which will affect the dynamic balance of the rotor.
We all know that each rotor of a centrifuge has a maximum speed and centrifugal force. The parameters should be set strictly according to the maximum centrifugal capacity of each rotor, otherwise it may lead to danger.
During the centrifugation process, the solution filling is unreasonable or the tank is not covered. The high vacuum state in the centrifuge chamber will cause the centrifuge tube to rupture and the sample liquid to overflow, resulting in the imbalance of the rotor and the occurrence of shaft bending or broken shaft accidents.
The maximum centrifugal force that different centrifuge tubes can withstand is different. If the centrifugal force exceeds the centrifuge tube, it may cause the tube to deform or even rupture.
The aging of the centrifuge tube or the use of the high-speed centrifuge centrifuge tube on the ultracentrifuge will cause the centrifuge tube to break during operation, and the sample liquid will overflow to cause imbalance and bend the shaft.
For centrifuge tubes of various materials, before use, they failed to carefully understand the scope of use of centrifuge tubes and disinfection methods required by the manufacturer, and used chemical solvents and inappropriate disinfectants to treat them, resulting in swelling and rupture of centrifuge tubes during operation and accidents.
Materials such as drive shafts and rotors have internal damage that has not been regularly detected, resulting in accidents in the centrifuge.
Improper use and improper disinfection of the rubber sealing ring in the centrifuge tube cap and the rubber sealing ring of the rotor cover, such as long-term use of internal fractures and high-temperature disinfection (baking in a drying oven), aging cracks and other defects lead to seal failure, and the sample is running at high speed. Time overflow, causing the rotor to run in an unbalanced state.
Due to carelessness in the work, the rotor cover is not tightened or the rotor cover and the rotor handle are interchanged, resulting in mismatched threads. When the machine is turned on, the rotor cover is thrown out and a serious shaft breaking accident occurs.
When the centrifuge tube is loaded with samples, because the sample liquid is not filled or the cap of the centrifuge tube is not tightly sealed, the high vacuum state in the centrifuge chamber during the centrifugation process will cause the centrifuge tube to deform, rupture and overflow the sample liquid, then the rotor is unbalanced and the shaft is bent or broken.
Accidents occur due to mixed use of pipe caps (the specific gravity of the two is different).
Due to improper service life management and various corrosion damages, etc., the rotor bursts during operation.
Due to carelessness, the fixing parts of the drive system or other systems were not removed during installation, and the instrument was forced to test run, resulting in broken shafts and rotor explosion accidents.