Disposable Cell Scrapers

In cell culture experiments, selecting suitable experimental equipment is crucial, especially when dealing with the subculture and harvesting of adherent cells. The disposable cell scrapers have become an indispensable tool. Disposable cell scrapers , with their unique design and safety features, can effectively reduce cell damage and ensure the smooth progress of the experiment. This article will introduce the unique design of the disposable cell scraper and its application in experiments in detail.

The Unique Design and Advantages of Disposable Cell Scrapers

Disposable cell scrapers are an important tool in tissue cell culture, designed with precision to minimize cell damage during operation. GongDong disposable cell scrapers  use medical-grade polypropylene material for the blade, which has high toughness and can reduce cell membrane damage when scraping adherent cells, making it particularly suitable for cell types that are difficult to digest.

Furthermore, the blade design of the disposable cell scrapers is flexible, and can rotate freely 360 degrees freely, which can easily reach every corner of culture flasks or dishes to ensure more comprehensive cell collection. The handle is available in 29cm and 25cm options to accommodate different specifications of culture containers. The independent paper-plastic packaging ensures sterility during use and convenience for laboratory personnel.

The features of being pyrogen-free, endotoxin-free, and non-cytotoxic also make the disposable cell scrapers one of the commonly used tools in laboratories. While ensuring the reliability of experimental results, it also provides safety for the operators.

Using the Disposable Cell Scrapers for J774A.1 Cell Digestion Operation

J774A.1  cells are a typical type of adherent cells that are difficult to digest with trypsin. Therefore, using the disposable cell scrapers  for handling these cells will be more convenient and efficient. Here are the specific steps for using the disposable cell scrapers  to handle J774A.1 cells:

Preparation of Materials:

• Disposable cell scrapers (unopened)

  
  

• Clean bench

  
  

• Centrifuge tubes, pipettes, and pipette guns (all sterilized and unopened)

Operating Procedures:

1. Pre-disinfection: Turn on the clean bench for 30 minutes for disinfection in advance, and the operators should undergo full-body disinfection according to sterile procedures.

2. Take out the materials: Take the J774A.1  cells from the incubator in a T25  culture flask, and place it with the disinfected disposable cell scrapers on the clean bench. Note that the culture flask should be placed upright and the cap should not be unscrewed in advance to prevent contamination.

3. Unpacking the scraper: Unpack the disposable cell scraper  package, hold the scraper with one hand, avoiding contact with the tabletop or other surfaces. Use the other hand to unscrew the culture flask cap, ensuring the inner side of the cap faces up to prevent contamination.

4. Scrape the cells: Insert the disposable cell scraper into the culture flask, adjust the blade direction along the cell-free wall, and gently scrape the adherent cells from outside to inside.

5. Complete the operation: After finishing the scraping, discard the cell scraper without immersing it into the culture medium. Use the pipette gun to gently blow down the cells on the flask wall and mix the cells with the culture medium, repeating 2-3 times.

6. Cell centrifugation: Transfer the cell and culture medium mixture into a centrifuge tube, then use a centrifuge for the centrifugation process. The next steps can follow the conventional passage or cryopreservation procedures.

Using the disposable cell scrapers can effectively solve the problem of difficult digestion of adherent cells while ensuring the integrity and safety of the cells. The entire operation process is convenient and fast, greatly improving experimental efficiency.