Application of Conical Flasks in Upstream Cell Culture Processes for Antibody Drugs

Antibody drugs are an important component of biological preparations, widely used in the treatment of tumors, management of autoimmune diseases, and other fields. The production process of antibody drugs involves multiple complex steps, with upstream processes, particularly cell culture, being key to determining the efficiency and quality of antibody drug production. Conical Flask is a commonly used laboratory consumable in cell culture that plays an important role, especially in the processes of cell resuscitation, expansion, and passage. This article will explore the specific application of Conical Flask in the upstream cell culture of antibody drugs.

Application of Conical Flask in Cell Resuscitation and Passage

In the upstream process of antibody drugs, cell resuscitation and passage are critical first steps. Thawing the frozen cell line from liquid nitrogen and placing it in a culture medium for cell resuscitation is a fundamental step in antibody drug production. After cell thawing, Conical Flask is commonly used for cell passage. Depending on the scale of the experiment, cells are often transferred to 125ml or 250ml Conical Flasks to maintain cell health and ensure a stable growth rate.

Conical Flask plays an important role in cell resuscitation and passage. Its smooth surface minimizes the risk of cell adhesion, ensuring an optimal environment for cell growth. Choosing high-quality Conical Flasks can effectively improve cell survival rates, accelerate the passage process, and help cells expand to meet the needs of subsequent large-scale culture. Therefore, the use of Conical Flasks in this stage is crucial, especially in enabling higher density cell growth and reducing the risk of contamination.

Application of Conical Flask in Seed Culture and Expansion

In the expansion phase of the upstream process, cells are passaged from small-scale Conical Flasks to larger containers. Typically, cells are expanded from the initial125ml or250ml Conical Flasks to 500ml or 1000ml Conical Flasks and are gradually transferred to larger seed tanks (such as 20L, 50L or 100L) to achieve the goal of cell expansion. This stage, known as seed culture, aims to increase the cell count to meet the demands of large-scale production.

Conical Flask also plays an irreplaceable role in seed culture and expansion. High-quality Conical Flasks materials, such as PETG or PC, have high transparency and durability, maintaining the stability of the cell culture environment. Additionally, the breathable membrane design of Conical Flasks increases air circulation, allowing cells to reach higher densities, thereby accelerating cell expansion. These characteristics support the smooth progress of the upstream cell culture process.

Role of Conical Flask in Large-Scale Cell Culture

After cells have expanded to a certain number, the next step is large-scale cell culture. This stage typically requires bioreactors for large-scale cell fermentation culture, while Conical Flasks provide a solid foundation for the growth of these cells in the early stages of passage and expansion culture. High-quality Conical Flasks ensure the health of cells in early culture stages, reducing the risk of adhesion or rupture during passage.

In the upstream process of antibody drugs, Conical Flasks are not only tools for cell passage but also play a role in optimizing the culture environment before expansion and large-scale cultivation. Their materials, design, and air permeability directly impact cell survival and growth throughout the entire process.