Requirements for Materials Used in Cell Culture Flask and Reasons for Cells Not Adhering to the Walls

What are the requirements for the material of cell culture flask?

Cell culture flasks are an economical and practical tool for cell culture. They are designed with a wide-necked shape, a frosted writing area, and optional breathable and sealable covers. To ensure cell growth, it is necessary to start at the source, which is the raw material.

Cell culture flasks generally use highly transparent polystyrene as the raw material. To ensure the safety of the raw material, it must comply with the USP Class VI medical grade standard. The US Pharmacopeia (USP) standardizes medical materials into six grades, ranging from USP class I to USP class VI, with USP class VI being the highest. According to the USP-NF regulations, plastics subjected to in vivo biological reaction testing will be categorized into designated medical plastics grades. The purpose of the test is to determine the biocompatibility of plastic products and whether they are suitable for medical device implants and other systems.

Being rated as the sixth class of US medical plastics means that comprehensive and rigorous testing has been established. US medical material class VI is now the gold standard for a wide range of medical-grade raw materials and an excellent choice for medical device manufacturers. Its testing items include systemic toxicity testing (in mice), intradermal reaction testing (in rabbits), and implantation testing (in rabbits).

Raw materials that comply with the USP VI standard are the basis for strict quality control of the cell culture flask. If the raw material contains a certain toxicity, it will not be conducive to cell growth and will affect the subsequent cell culture experiments.

What are the reasons for the cells not adhering to the wall in cell culture flask?

Adherent culture is a common way of culture. In general, cell culture flasks and other cell consumables will be used, and cells will adhere to the surface of the cell culture flask for proliferation. However, sometimes cells do not adhere to the wall. What is the reason for this? Gongdong, the excellent medical oem supplier, will take you on a quick journey into the world of e-bike motors. 

Analysis of the reasons why cells do not adhere to the wall

• Poor control of the digestion time of trypsin: If the digestion is not sufficiently fine, the cells themselves will aggregate. If trypsin is processed for too long, it will easily cause damage to the cell membrane protein, making the cell adhesion loose and the stereoscopic feeling not strong under the microscope. In severe cases, it may even cause cell death.

• Lack of adhesion factors: Serum contains factors that promote adhesion, and in serum-free culture medium, the adhesion effect of cells will decrease significantly due to the lack of these adhesion factors.
  

• Contamination with mycoplasma or bacteria.
  

• The revived cells themselves are in poor condition, aging, and have lost adhesion.
  

• The number of seeded cells is too small, and they cannot secrete enough extracellular matrix.
  

The above are several common reasons for cells not adhering to the wall when using cell culture flasks and other containers. Specific treatments can be carried out according to the specific situation, enabling the cells to be cultured by adhesion.