Sterilization Methods and Selection Factors for Cell Culture Flask

Cell culture technology is widely used in various disciplines such as cytology, genetics, immunology, experimental medicine, and oncology. When culturing cells, there are strict requirements for operating procedures and consumable selection. Cell culture flasks, as commonly used disposable consumables, should follow what selection principles?

Introduction of sterilization methods for cell culture flask

Cell culture refers to a method of simulating the in vivo environment in vitro, allowing cells to survive, grow, reproduce, and maintain their main structure and function, with one very important condition being aseptic. It requires that the environment for cell culture must be aseptic, and the cell culture flasks and other culturing tools involved also need to meet aseptic requirements and undergo sterilization treatment.

Among many sterilization methods, a cell culture flask mainly uses cobalt-60 sterilization, which is the use of 60Co γ-rays to affect microorganisms, directly or indirectly destroying the microorganisms' cell nucleus, thereby killing microorganisms and achieving disinfection and sterilization. It is a type of radiation sterilization technology, where gamma rays produced by cobalt-60 radioactive isotopes are used to irradiate products. In the transfer and conversion of energy, gamma rays create powerful physical and biological effects, achieving the purpose of insecticidal, bactericidal, and inhibiting physiological processes.

60Co-γ ray irradiation sterilization is a special "cold working" technology, which sterilizes at room temperature. The high energy and strong permeability of γ rays do not pollute the environment and leave no residue, resulting in thorough sterilization effects. While sterilizing, it does not raise the internal temperature of the product, hence being referred to as "cold" sterilization method.

After completing the cobalt-60 sterilization, cell culture flasks can meet the aseptic requirements, avoiding contamination of the later stage cells caused by various microorganisms carried by culture containers, which may affect the experimental process. Gongdong is a professional medical oem supplier, and if you are interested in cell culture flasks wholesale, please contact us for more information.

Factors for selecting cell culture flask

Raw materials for the production of cell culture flask

Cell culture flasks generally use polystyrene as raw material, which are processed by injection molding. When selecting consumables, we should consider whether they meet the USPVI medical grade raw material requirements. Quality raw materials can minimize the risk of elution.

Processing technology for cell culture flask

These consumables are generally used for culturing adherent cells, but their material surface is hydrophobic, and must undergo special surface treatment to change its performance, to ensure that the cells can attach to its surface and grow well.

Quality assurance for cell culture flask

A high-quality product must be strictly controlled in terms of raw materials, production processes, testing, internal and external packaging, etc. When selecting consumables, we should also see whether the manufacturer has a complete quality assurance system.

Cost performance of cell culture flask

After comprehensively considering the above factors, then consider the cost performance of the product. Why not reduce costs while ensuring product performance?

These are the four factors to consider when selecting cell culture flasks. In addition, if the procurement volume is large, we should also see whether the manufacturer's supply capacity is stable to avoid affecting later use.