When cultivating cells, we often use cell culture flasks, which come in various sizes ranging from 25cm² to 175cm² to meet different cell culture needs. Depending on the cell culture method used, this type of container has two different surfaces: TC-treated and non-TC-treated.
Cells are divided into adherent cells and suspension cells depending on the culture method, and the growth of adherent cells must have a support surface that they can adhere to. Cells rely on attachment factors secreted by themselves or provided in the culture medium to grow and multiply on this surface. Suspension cells do not require a support surface, and grow in a suspended state in the culture medium, such as lymphocytes.
Cell culture flasks are made of polystyrene, which is hydrophobic and not conducive to cell adhesion. Therefore, it needs to undergo surface TC treatment to change its properties so that the cells can adhere better to it. Suspension cell culture containers do not have this requirement, but TC-treated containers are generally suitable for suspension cell culture.
In simple terms, a cell culture flask with TC-treated surfaces is more suitable for adherent cell culture, while containers without TC treatment are suitable for suspension cell culture. When selecting a cell culture container, we must first determine the cell culture method, and then choose the appropriate container to ensure the good growth of cells and the smooth progress of experiments.
In adherent cell culture, cell culture flasks are a commonly used container with a variety of sizes and clever designs to meet different cell culture needs. Have you noticed its three user-friendly designs when using this container? As a professional medical oem supplier, we will introduce this in detail:
In cell culture, the culture medium is an indispensable solution that provides the necessary nutrients for cell growth. Depending on the culture scale, the amount of culture medium added may vary. How do we control the adding volume? This container has a high-definition scale on the side, which helps us to estimate the volume of the culture medium conveniently and quickly.
In actual cell culture operations, we also use consumables such as pipettes and cell scrapers. Whether it is transferring solution or scraping cells from the bottom, it needs to be in perfect contact with the flask. This container adopts a angled and super wide neck design, making it easier for cell scrapers or pipettes to enter the growth surface for easier operation.
Frosted writing area
Have you ever mixed up cells? To facilitate the recording of operators, there is a frosted writing area on the neck of the flask, so we can clearly record information such as cell types and time, and avoid mixing up cells.
These are the three user-friendly designs of cell culture flasks, which meet various needs of cell culture experiments from the perspective of operators, and are also basic requirements for cell culture containers.