Serum Separation Tubes

When using a serum separator tube, collect the specimen using standard venipuncture technique. Gently invert the tube five times to mix the clot activator with the blood. Allow the tube to clot for 20-30 minutes in a vertical position. Centrifuge the tube for 10-15 minutes at about 1300-1800 xg. The centrifuge RPMs will differ depending on the centrifuge and rotor used. The gel barrier will develop, keeping the serum and clot apart.

The Serum Separator tube is used when they want to test the serum of the blood. For tests like (NA, K, CL, CO2, LD, HDL) any type of chemistry. It is also used for tests like neonatal bilirubin or an AFP non maternal. There is special gel that separates blood cells from serum, as well as particles to cause blood to clot quickly. The blood sample may then be centrifuged, allowing the clear serum to be removed for testing.