Cell culture plates can be divided into flat-bottom and round-bottom (U-shaped and V-shaped) according to the shape of the bottom; the number of culture wells can range from 6, 12, 24, 48, 96 to 384 and 1536, depending on the material, there are Terasaki plates and regular cell culture plates. The specific choice depends on the type of cultured cells, the required culture volume and different experimental purposes.
Cell culture plates of different shapes have different purposes. Usually, flat-bottom plates are used for cell cultivation because they are easy to observe under a microscope, have a clear bottom area, and a relatively consistent height of the cell culture solution. Therefore, for experiments like MTT, both adherent and suspended cells usually use flat-bottom plates. When measuring absorbance values, flat-bottom culture plates must be used. Attention should be paid to the material, where "Tissue Culture (TC) Treated" indicates that it is used for cell cultivation.
U-shaped or V-shaped plates are generally only used when there are special requirements. For example, in immunology, when two different lymphocytes are mixed for cultivation, they need to contact and stimulate each other. At this time, U-shaped plates are generally used because the cells will be gathered in a small range due to gravity. Round-bottom culture plates are also used in experiments involving isotope incorporation, such as "mixed lymphocyte cultures," where cells need to be collected using a cell collector. V-shaped plates are commonly used for cell killing and immunological agglutination experiments. U-shaped plates can also be used as alternatives for cell killing experiments (after adding cells, centrifuge at low speed).
Terasaki plates are mainly used in crystallographic research, and their design is convenient for observing and analyzing the structure of crystals. There are two methods, sitting and handing drop, and the shape and structure of the product also differ according to the two methods. Crystal class polymer is chosen for materials, and special materials are advantageous for observing the structure of crystals.
This type of cell culture plate is mainly made of USP VI grade PS material, with a treated surface that is suitable for the adhesion and extension of cells. Of course, there are also materials for the growth of floating cells, as well as for low binding surfaces.
ELISA plates are generally more expensive than cell culture plates. Cell culture plates are mainly used for cell culture and can also be used to measure protein concentration; ELISA plates include the capture plate and reaction plate, which are not generally used for cell culture but for protein detection after immunoenzymatic reactions, requiring higher requirements and specific enzyme-linked working fluids.
The liquid level of the culture solution added to different wells should not be too deep, generally ranging from 2 to 3 mm. Combining the bottom area of each well, the suitable liquid volume of each culture well can be calculated. Too much liquid can affect gas (oxygen) exchange and can easily overflow during transportation, causing contamination. The specific cell density to be added should be flexibly controlled according to different experimental purposes.