Views: 0 Author: Site Editor Publish Time: 2025-03-03 Origin: Site
With the continuous advancement in biological research, mastering experimental skills is becoming increasingly important. In the experimental process, the usage method of disposable cell scrapers, as a commonly used tool, should not be underestimated for its impact on experimental results. This article will provide a detailed introduction to the usage method of disposable cell scrapers, helping you easily master biological experimental skills.
• Types and Selection of Disposable Cell Scrapers
Disposable cell scrapers are mainly divided into manual scrapers and electric scrapers. Manual scrapers are easy to operate but require significant force; electric scrapers can reduce labor intensity but are relatively more expensive. When choosing a disposable cell scraper, you should select based on your actual needs and economic conditions.
• Disinfection and Maintenance of Cell Scrapers
• Disinfection Methods
• Alcohol Disinfection
Soak the disposable cell scraper in a 75% alcohol solution for no less than 30 minutes.
• Boiling Disinfection
Place the disposable cell scraper in boiling water for 15-20 minutes.
• Ultraviolet Disinfection
Place the disposable cell scraper under a UV lamp for more than 30 minutes.
• Maintenance Methods
1. Rinse immediately with clean water after use to avoid residual substances affecting the next usage.
2. Wipe dry the moisture with a soft cloth or paper towel to prevent bacterial growth from the moisture.
3. Regularly disinfect and maintain the disposable cell scraper to ensure its cleanliness and hygiene.
• Usage Techniques of Disposable Cell Scrapers
• Preparatory Work
Before using the disposable cell scraper, firstly prepare the following items: cell culture dishes, cell culture medium, sterile gloves, etc. Also, ensure that the cell culture dishes and cell culture medium used are sterile.
• Sampling Operation
Before sampling, make sure to wear sterile gloves to prevent infection. During sampling, try to avoid damaging the cell surface. If a large area of cells needs to be sampled, multiple small containers can be used for distribution.
• Scraping Operation
1. Hold the handle of the disposable cell scraper, keeping the blade close to the skin at a certain angle.
2. Pinch the handle with the thumb and index finger, tilting the blade at a 45-degree angle.
3. Gently scrape along the cell surface with moderate force, ensuring not to damage the cell surface.
4. After scraping, gently wipe the remaining cell fragments with a sterile cotton swab.
• Handling Operation
1. Place the sampled cell culture dishes in a sterile box for subsequent experiments.
2. Pour the remaining cell culture medium into the waste liquid tank for harmless treatment.
As a commonly used tool in biological experiments, the usage method of disposable cell scrapers has a significant impact on experimental results. By mastering the correct usage methods and maintenance techniques, we can better conduct biological experiments, improving experimental efficiency and accuracy. I hope this article can be helpful to everyone. Let's work together to explore the mysteries of biology!